A Study on an Automatic Multi-Focus System for Cell Observation


Jaeyoung Park, Sangjoon Lee, Journal of Information Processing Systems
Vol. 15, No. 1, pp. 47-54, Feb. 2019
10.3745/JIPS.04.0099
Keywords: Automatic Multi-Focus, Cell Observation, Cubic Spline Interpolation, Peak Detection
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Abstract

This study is concerned with the mechanism and structure of an optical microscope and an automatic multifocus algorithm for automatically selecting sharp images from multiple foci of a cell. To obtain precise cell images quickly, a z-axis actuator with a resolution of 0.1 ?m was designed to control an optical microscope Moreover, a lighting control system was constructed to select the color and brightness of light that best suit the object being viewed. Cell images are captured by the instrument and the sharpness of each image is determined using Gaussian and Laplacian filters. Next, cubic spline interpolation and peak detection algorithms are applied to automatically find the most vivid points among multiple images of a single object. A cancer cell imaging experiment using propidium iodide staining confirmed that a sharp multipoint image can be obtained using this microscope. The proposed system is expected to save time and effort required to extract suitable cell images and increase the convenience of cell analysis.


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Cite this article
[APA Style]
Jaeyoung Park and Sangjoon Lee (2019). A Study on an Automatic Multi-Focus System for Cell Observation. Journal of Information Processing Systems, 15(1), 47-54. DOI: 10.3745/JIPS.04.0099.

[IEEE Style]
J. Park and S. Lee, "A Study on an Automatic Multi-Focus System for Cell Observation," Journal of Information Processing Systems, vol. 15, no. 1, pp. 47-54, 2019. DOI: 10.3745/JIPS.04.0099.

[ACM Style]
Jaeyoung Park and Sangjoon Lee. 2019. A Study on an Automatic Multi-Focus System for Cell Observation. Journal of Information Processing Systems, 15, 1, (2019), 47-54. DOI: 10.3745/JIPS.04.0099.